Pioneering Instrumentation for Transient Kinetics & Spectroscopy

Photo Diode Array

Product code: MG6560

*This product is no longer available and has been replaced by the new MG7000 KinetaScan CCD.

Click on CCD Detector for more information.

 

 

 

This approach is very efficient in terms of generating much information from limited quantities of reagents - all wavelengths are observed simultaneously. The KinetaScan is a high performance system with better than 2 nm resolution and capable of collecting spectral data between 280 nm and 700 nm (shifted wavelength versions are available). Under most circumstances spectra are collected at 1.5 ms integration time thus providing valuable information even for relatively fast kinetic events.

Key to the performance of the KinetaScan are the well designed optics on all KinetAsyst systems and the custom software control package which is incorporated into the Kinetic Studio control software.

This software control enables spectral calibration and the setting of reference levels. Data collection is initiated by the stopping event and spectra are collected, normalised and displayed in true absorbance units. Kinetic Studio allows the collection of spectra with an integration time of as little of 1.25 ms. A logarithmic time base is also available for the study of multi-phasic reactions with both fast and slow phases. Integration times can be adjusted for low light levels or slower reactions. Spectral series can be processed to yield time domain plots either directly or by singular value decomposition and global analysis with tools such as ReactLab and SPECFIT/32.

An automatic shutter option is available for this product which can be used to reduce any unwanted effects from incident light on longer run times - simply, it protects the sample from light between scans.

The diode array is available for all KinetAsyst Stopped-Flow systems as well as High Pressure and CryoStopped-Flow systems.

Selected Publications

  1. Winter, M.B., Herzik Jr., M.A., Kuriyan, J. and Marletta, M.A. (2011) Tunnels modulate ligand flux in a heme nitric oxide/oxygen binding (H-NOX) domain. PNAS, 108, no. 43, pp. E881-E889.
  2. Tejero, J., Biswas, A., Haque, M.M., Wang, Z-Q., Hemann, C., Varnado, C.L., Novince, Z., Hille, R., Goodwin, D.C. and Stuehr, D.J. (2011) Mesohaem substitution reveals how haem electronic properties can influence the kinetic and catalytic parameters of neuronal NO synthase. Biochem. J. 433, pp. 163-174.
  3. Jin, N., Lahaye, D.E. and Groves J.T. (2010) A "Push-Pull" Mechanism for Heterolytic O-O Bond Cleavage in Hydroperoxo Manganese Porphyrins. Inorg. Chem. 49, pp. 11516-11524.
  4. Smieja, J.M., Benson, E.E., Kumar, B., Grice, K.A., Seu, C.S., Miller, A.J.M., Mayer, J.M. and Kubiak, C.P. (2012) Kinetic and structural studies, origins of selectivity, and interfacial charge transfer in the artificial photosynthesis of CO PNAS, 109, no. 39, pp. 15646-15650. (An example using Cryo stopped-flow).
  5. Bruckner, R.C. and Jorns, M.S. (2009) Spectral and Kinetic Characterization of Intermediates in the Aromatization Reaction Catalyzed by NikD, an Unusual Amino Acid Oxidase. Biochemistry, 48(21), pp. 4455-4465.