Pioneering Instrumentation for Transient Kinetics & Spectroscopy

Stopped-Flow for FT-IR

Product Code: SF-61/FT-IR

Although UV-visible spectroscopic methods are well suited to stopped-flow methods and are hence firmly established as a method of choice, vibrational spectroscopy provides more structural information in terms of bonds and mobility.

Although more complex to implement, TgK Scientific in combination with established suppliers of FT-IR instruments have produced a stopped-flow solution with a short path length cell. Latest Software: KinetaDrive 1.04.

Bruker have more information on their web site regarding their range of VERTEX instruments and how they can be used with the TgK FT-IR Stopped-Flow system.

Link: Bruker

Selected References

  1. Wright, J.A., Webster,L., Jablonskyte, A., Woi, P.M., Ibrahim, S.K. and Pickett, C.J. (2011) Protonation of [FeFe]-hydrogenase sub-site analogues: revealing mechanism using FTIR stopped-flow techniques. Faraday Discuss.,148, pp. 359-371.
  2. Jablonskyte, A., Wright, J.A. and Pickett, C.J. (2010) Mechanistic aspects of the protonation of [FeFe]-hydrogenase subsite analogues. Dalton Trans.,39, pp. 3026-3034.
  3. Wright, J.A. and Pickett, C.J. (2009) Protonation of a subsite analogue of [FeFe]-hydrogenase: mechanism of a deceptively simple reaction revealed by time-resolved IR spectroscopy. Chem. Commun., pp. 5719-5721.

Application Note

Monitoring enzyme catalysis using the VERTEX 80 FT-IR spectrometer in Rapid Scan mode

Abstract α-chymotrypsin is a well characterised mammalian digestive enzyme that catalyses the hydrolytic cleavage of peptide bonds at the carboxyl side of aromatic residues. During the chymotrypsin-catalysed hydrolysis of N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (Suc-AAPF-pNA; see figure 1), formation of the p-nitroaniline product can be followed spectroscopically at 410 nm while the peptide product can be monitored by FT-IR spectroscopy due to formation of a new C-terminal carboxylate group. The VERTEX 80 FT-IR spectrometer with the UltraScanTM linear air bearing scanner with True-Alignment technology is ideally suited for such kinetic studies, since at the fastest mirror velocity (320 kHz) more than 100 spectra at resolution 16 cm-1 can be collected per second.

Link to the full application note: Bruker application note

Information about the Bruker VERTEX 80 FT-IR spectrometer: Brochure

For more detailed information on the TgK Scientific FT-IR Stopped-Flow sample delivery system, please contact us.