Rapid Quench Flow Systems
Product codes: RQF-63 and RQF-66
The RQF Series of instruments uses the rapid quench flow technique to provide a powerful method of studying rapid reactions that cannot be monitored by absorbance or fluorescence spectrophotometry. Furthermore, this allows the use of analytical techniques to be used that can specifically determine reaction intermediates.
These instruments feature a sample handling unit that contain a biochemical compatible thermostated flow circuit. An associated electronics control unit determines both the volume delivered into the system and its flow rate, enabling age times to be set from <5 ms to 100 s. A wide range of age times can be realised since both systems can be operated in continuous and push-push or "interrupt" mode.
For continuous mode, a range of age loops is supplied. These are mounted on cartridges for easy exchange without any disruption of the thermostat system. For the interrupt mode, only one of the age loops is used. The two reagents are mixed together and held in the loop, and after a predetermined delay set at the controller, the drive delivers a second push emptying and quenching the reaction volume.
Our extensive experience in the handling of small volumes of solution for kinetics studies has led to a modular sample circuit design, virtually eliminating discrete tubing and connectors. The solution displacement syringes are driven by a single powerful stepper motor thus delivering precise and known flow rates with excellent reproducibility.
