Pioneering Instrumentation for Transient Kinetics & Spectroscopy

Rapid Quench Flow

Product Code: RQF-63

The RQF-63 Quench-Flow sample handling unit features a biochemical compatible thermostated flow circuit. A dedicated electronics control unit sets the volume delivered into the system and its flow rate. Age times can be defined from <5 ms to 100 s by operating the instrument in continuous, 'push-push' or 'interrupt' mode.

For continuous mode, a range of age loops are supplied. These are mounted on cartridges for easy exchange without any disruption of the thermostat system. For the interrupt mode, just one of the age loops is necessary. The two reagents are mixed together and held in the loop, and after a predetermined delay set by the controller, the drive delivers a second push emptying and quenching the reaction volume.


Our extensive experience in the handling of small volumes of solution for kinetics studies has led to a modular sample circuit design, virtually eliminating discrete tubing and connectors. The solution displacement syringes are driven by a single powerful stepper motor thus delivering precise and known flow rates with excellent reproducibility.

We have experience of installing Quench-Flow instruments in glove boxes for strictly anaerobic operation.

Selected Publications

  1. Barman, T.E., Bellamy, S.R.W., Gutfreund, H., Halford, S.E. and Lionne, C. (2006) The identification of chemical intermediates in enzyme catalysis by the rapid quench-flow technique. Cell. Mol. Life Sci. 63 (2006) pp. 2571-2583.
  2. Tongsook, C., Sucharitakul, J., Thotsaporn, K. and Chaiyen, P. (2011) Interactions with the Substrate Phenolic Group are Essential for Hydroxylation by the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase. Journal of Biological Chemistry, 286(52), pp. 44491-502.
  3. Ruangchan, N., Tongsook, C., Sucharitakul, J., and Chaiyen, P. (2011) pH-dependent studies reveal an efficient hydroxylation mechanism of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. Journal of Biological Chemistry, 286(1), pp. 223-33.

Application Notes

AN.005.R63

Rapid quench-flow studies of enzymatic cleavage of a covalently closed circular DNA.

AN.024.QF63

Rapid quench-flow temporal calibration introducing a Windows application to control the sample delivery.